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Cathepsin D Mouse mAb - Primary antibody, specific to Human Cathepsin D

    Application:
  • ELISA
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: AB04/3H12
  • Conjugation: Unconjugated
In stock
Item Number
Ab225221
Grouped product items
SKU Size
Availability
Price Qty
Ab225221-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$52.90
Ab225221-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$159.90
Ab225221-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab225221-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,799.90

mAb (AB04/3H12); Mouse anti Human CTSD; WB, Flow Cytometry, ELISA; Unconjugated

Basic Description

Product Name Cathepsin D Mouse mAb - Primary antibody, specific to Human Cathepsin D
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzhei
Host species Mouse
Specificity Human Cathepsin D
Immunogen Recombinant protein corresponding human Cathepsin D
Positive Control WB: MCF-7 and A431 whole cell lysates Flow Cytometry: MCF-7 cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Cathepsin D Antibody, clone AB04/3H12, could be used for WB, Flow Cytometry, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CTSD Tchem Cathepsin D (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Cathepsin D Mouse mAb (Ab225221) - Western Blot
All lanes: Cathepsin D Mouse Antibody (Ab225221) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 29, 45 kDa
Exposure time: 30.0 s 

Cathepsin D Mouse mAb (Ab225221) - Flow Cytometry ​
Overlay histogram showing MCF-7 cells stained with Cathepsin D Mouse mAb (Ab225221) (Red) at 1.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).​

Application

Application Dilution info
WB

1/1000 - 1/100000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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