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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab225221-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$52.90
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Ab225221-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$159.90
|
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Ab225221-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$249.90
|
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Ab225221-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,799.90
|
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mAb (AB04/3H12); Mouse anti Human CTSD; WB, Flow Cytometry, ELISA; Unconjugated
| Product Name | Cathepsin D Mouse mAb - Primary antibody, specific to Human Cathepsin D |
|---|---|
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzhei |
| Host species | Mouse |
| Specificity | Human Cathepsin D |
| Immunogen | Recombinant protein corresponding human Cathepsin D |
| Positive Control | WB: MCF-7 and A431 whole cell lysates Flow Cytometry: MCF-7 cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Cathepsin D Antibody, clone AB04/3H12, could be used for WB, Flow Cytometry, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Cathepsin D Mouse mAb (Ab225221) - Western Blot
All lanes: Cathepsin D Mouse Antibody (Ab225221) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 29, 45 kDa
Exposure time: 30.0 s
Cathepsin D Mouse mAb (Ab225221) - Flow Cytometry
Overlay histogram showing MCF-7 cells stained with Cathepsin D Mouse mAb (Ab225221) (Red) at 1.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/100000 |