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Recombinant EN-TEV Protease Protein, ＞98% (SDS-PAGE), high purity
- Expression System: E. coli
- Accession #: P04517
- Protein Tag: N-His
- Bioactivity: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
- Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp156465
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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rp156465-1KU
|
1KU |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$199.90
|
|
|
rp156465-10KU
|
10KU |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$1,299.90
|
|
Carrier free, >98% (SDS-PAGE), Active, E.coli, N-His tag, 2038-2279 aa