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Casein-FITC conjugate

COA

Grade & Purity:

Ex:498nm

Em:517nm

In stock

Item Number

C275894

Grouped product items
SKU	Size	Availability	Price
C275894-5mg	5mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$129.90
C275894-25mg	25mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$259.90
C275894-100mg	100mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$799.90

Conjugated protein substrate used for fluorometric measurement of protease activity in vitro

View related series

Casein (4) Casein-FITC (1) FITC (181)

Basic Description

Product Name	Casein-FITC conjugate
Synonyms	FITC-Casein \| fluorescein isothiocyanate (FITC)-labeled casein
Grade	Em:517nm, Ex:498nm
Product Description	Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.
Specifications & Purity	Ex:498nm, Em:517nm
Conjugation	FITC
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	517nm
Ex/Em(nm)
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Storage and Shipping

Storage Temp	Store at 2-8°C,Protected from light
Shipped In	Wet ice
Stability And Storage	Product should be stored at 2-8℃ in the dark. After reconstitution, store in aliquots and freeze material at -20℃, avoid freeze/thaw cycle.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

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