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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab191653-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$49.90
|
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Ab191653-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$95.90
|
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Ab191653-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$216.90
|
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mAb (2B8); Rat anti Mouse c-Kit/CD117 Antibody (AF488); Flow Cytometry
| Product Name | c-Kit/CD117 Rat mAb (AF488) - Primary antibody, specific to c-Kit/CD117 |
|---|---|
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration a |
| Host species | Rat |
| Specificity | c-Kit/CD117 |
| Immunogen | Mouse bone marrow mast cells |
| Positive Control | Flow: C57BL/6 mouse bone marrow cells. |
| Conjugation | AF488 |
| Excitation(nm) | Blue Laser(488nm) |
| Emission(nm) | 525nm |
| Ex/Em(nm) | Ex:490nm;Em:525nm |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse c-Kit/CD117 Antibody, Monoclonal (2B8), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
c-Kit/CD117 Rat mAb (AF488) (Ab191653) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit/CD117 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (AF488) (Ab190240; Left panel) or c-Kit/CD117 Rat mAb (AF488) (Ab191653; Right panel) at 0.5μg/test.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |