Basic Description

Synonyms	1,3-Bis[tris(hydroxymethyl)methylamino]propane \| Bis-Tris propane \| bis(2-(hydroxymethyl)propane-1,3-diol) \| 2,2'-(Propane-1,3-diylbis(azanediyl))
Specifications & Purity	≥99%
Biochemical and Physiological Mechanisms	Buffer that is useful in the pH 6.3-9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close.
Storage Temp	Argon charged
Shipped In	Normal
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments. Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III). Product Application： BIS-TRIS Propane may be used in the following studies: as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2). for purification of glucose-binding protein from membranes of Sulfolobus solfataricus. as a buffer during GeO2 mineralisation. as a buffer component in a study pertaining to environmental research as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organic nitrogen compounds
  - Class Organonitrogen compounds
    - Subclass Amines
      - Level5 Alkanolamines
        Level6 1,2-aminoalcohols

Kingdom	Organic compounds
Superclass	Organic nitrogen compounds
Class	Organonitrogen compounds
Subclass	Amines
Intermediate Tree Nodes	Alkanolamines
Direct Parent	1,2-aminoalcohols
Alternative Parents	Dialkylamines Primary alcohols Organopnictogen compounds Hydrocarbon derivatives
Molecular Framework	Aliphatic acyclic compounds
Substituents	1,2-aminoalcohol - Secondary amine - Secondary aliphatic amine - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Primary alcohol - Organooxygen compound - Alcohol - Aliphatic acyclic compound
Description	This compound belongs to the class of organic compounds known as 1,2-aminoalcohols. These are organic compounds containing an alkyl chain with an amine group bound to the C1 atom and an alcohol group bound to the C2 atom.
External Descriptors	hexol
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Names and Identifiers

Pubchem Sid	488187786
Pubchem Sid Url	https://pubchem.ncbi.nlm.nih.gov/substance/488187786
IUPAC Name	2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol
INCHI	InChI=1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
InChIKey	HHKZCCWKTZRCCL-UHFFFAOYSA-N
Smiles	C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
Isomeric SMILES	C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
WGK Germany	3
Molecular Weight	282.33
Beilstein	1786109
Reaxy-Rn	1786109
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1786109&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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28 results found

Lot Number	Certificate Type	Date	Item
F2405224	Certificate of Analysis	Jun 15, 2024	B105640
F2405225	Certificate of Analysis	Jun 15, 2024	B105640
F2405226	Certificate of Analysis	Jun 15, 2024	B105640
D2408114	Certificate of Analysis	Apr 18, 2024	B105640
D2412184	Certificate of Analysis	Apr 15, 2024	B105640
B2422072	Certificate of Analysis	Feb 20, 2024	B105640
B2426412	Certificate of Analysis	Feb 20, 2024	B105640
K2321045	Certificate of Analysis	Nov 29, 2023	B105640
K2321046	Certificate of Analysis	Nov 29, 2023	B105640
K2321047	Certificate of Analysis	Nov 29, 2023	B105640
D2303525	Certificate of Analysis	Feb 20, 2023	B105640
D2303524	Certificate of Analysis	Feb 20, 2023	B105640
C2303411	Certificate of Analysis	Feb 20, 2023	B105640
C2303412	Certificate of Analysis	Feb 20, 2023	B105640
C2303413	Certificate of Analysis	Feb 20, 2023	B105640
B2303881	Certificate of Analysis	Feb 18, 2023	B105640
B2303882	Certificate of Analysis	Feb 16, 2023	B105640
B2303883	Certificate of Analysis	Feb 13, 2023	B105640
D2303522	Certificate of Analysis	Feb 13, 2023	B105640
D2303523	Certificate of Analysis	Feb 13, 2023	B105640
A1925024	Certificate of Analysis	Nov 14, 2022	B105640
J2222081	Certificate of Analysis	Oct 25, 2022	B105640
J2222082	Certificate of Analysis	Oct 25, 2022	B105640
J2222083	Certificate of Analysis	Oct 25, 2022	B105640
F2213229	Certificate of Analysis	Jun 15, 2022	B105640
F2213241	Certificate of Analysis	Jun 15, 2022	B105640
F2213193	Certificate of Analysis	Jun 15, 2022	B105640
F2213182	Certificate of Analysis	Jun 15, 2022	B105640

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Chemical and Physical Properties

Solubility	Soluble in water
Sensitivity	Moisture sensitive
Melt Point(°C)	164-165°C
Molecular Weight	282.330 g/mol
XLogP3	-4.600
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	12
Exact Mass	282.179 Da
Monoisotopic Mass	282.179 Da
Topological Polar Surface Area	145.000 Å²
Heavy Atom Count	19
Formal Charge	0
Complexity	183.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Documents & Articles

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Citations of This Product

How to Cite Aladdin Scientific Products?

1. Jie Gu, Shuyun Zheng, Xiaolong Lu, Hao Zhang, Kai Ren, Ziqiang Liu, Chao Liu, Chunrui Wu. (2024) A new strategy for solving hydrophobic membrane wetting: membrane structure design for membrane pore drying by spontaneous dehydration. JOURNAL OF MEMBRANE SCIENCE, (123664).
2. Jiandong Shen, Bijiang Zhong, Wenshui Xia, Yanshun Xu. (2024) Action of structural proteins in textural deterioration of grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 59 (4): (2659-2666).
3. Seokjoon Kim, Seungjin Lee, Seokhwan Kim, Jiye Shin, Byung Seok Cha, Eun Sung Lee, Ki Soo Park. (2024) Colorimetric detection of arsenite using Tris-mediated gold nanoparticle aggregation and chitosan lateral flow strip-based signal enhancement. SENSORS AND ACTUATORS B-CHEMICAL, 407 (135469).

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SKU	Size	Availability	Price
B105640-5g	5g	4	$15.90
B105640-25g	25g	9	$55.90
B105640-100g	100g	7	$156.90
B105640-500g	500g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$549.90

BIS-TRIS propane - 99%, high purity , CAS No.64431-96-5