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Basic Description
| Synonyms | Antonia Red™-lysine-dextran | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Storage Temp | Room temperature,Argon charged | |||||||||||||||||||
| Shipped In | Normal | |||||||||||||||||||
| Product Description |
mesenteroides labelled with Lysine. Lysine dextran is then functionalized with Antonia Red under mild conditions to form Antonia Red Lysine Dextran. After purification, the products are controlled for Mw, appearance, solubility, DS, pH, free Lysine and free dye. The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product Antonia Red Lysine Dextran 4 has a molecular weight of approx. 4000 Da. The actual molecular weight is determined by GPC. This value is supplied with the Certificate of Analysis. The dextran is essentially a linear α-(1-6)-linked glucose chain with however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from Mw of 4000 to 500000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters.Physical propertiesAntonia Red is a novel red-shifted dye similar to Texas Red and is developed and produced by Aladdin Consultancy AB. Antonia Red Lysine Dextrans are purple powders that are readily soluble in water or electrolyte solutions. Antonia Red Lysine Dextran are insoluble in most organic solvents, such as ethanol, methanol, acetone, chloroform, ethyl acetate etc. The degree of substitution, (DS) is between 0.01-0.02 (mol Lysine/mol Glucose) and 0.001-0.01 (mol Antonia Red/mol Glucose).Spectral data Antonia Red-lysine-dextran has an absorbance maximum at 583nm and an emission wavelength of 602 nm in borate buffer at pH 9.0.Stability No prospective stability studies on Antonia Red Lysine Dextrans have been performed yet. However, the structural properties of the dextran and of the carbamide linkage of the Lysine to the dextran chain as well as the convalent bond between Lysine and Antonia Red would suggest high stability of the product. It is recommended that the products are stored in air-tight containers. Antonia Red Lysine Dextrans may be stored at ambient temperatures. Application Antonia Red-lysine-dextrans offer easy conjugation for biomolecules and may be used for preserving cells in a” life like” state. Antonia Red shows bright fluorescence, has good photo- and chemical stability and is non-pH dependent.Dextrans carrying amino groups are valuable to the scientific community as versatile tools for bioconjugation and fixation in living systems1. For conjugation, the free amino group of the Lysine structure will be able to covalently bind to activated entities such as NHS-esters or isothiocyanates or be coupled to carboxylic acids using common peptide coupling reagents such as DCC, HOBt and or HATU. Cell and tissue fixation is performed in order to preserve components in a “life-like state” and to make cells permeable to allow antibodies to access cellular structures, for example for microscopy studies or immunostaining. The amino groups on Lysine Dextran reacts with the fixing agent (here: formaldehyde or glutaraldehyde) to form a covalent crosslinking with biomolecules such as proteins and lipids, immobilizing the whole system and the dextran. This is of particular importance when evaluating biological events qualitatively or quantitatively in molecular imaging. Without fixation, these structures within a living system would fall apart and diffuse rapidly. Antonia Red is a fluorescent dye that has an excitation maximum at 583 nm and an emission maximum at 611 nm, making it an analogue of the popular Texas Red dye. Product specifications and parameters：
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Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 F2310765 |
Certificate of Analysis | Jun 25, 2023 | A491542 |
| F2310766 |
Certificate of Analysis | Jun 25, 2023 | A491542 |
| E2317397 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317390 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317399 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317398 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317402 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317395 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317394 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317401 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317388 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317391 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317387 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2527021 |
Certificate of Analysis | May 26, 2023 | A491542 |
| G2501013 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317405 |
Certificate of Analysis | May 26, 2023 | A491542 |
| E2317393 |
Certificate of Analysis | May 25, 2023 | A491542 |
| E2317384 |
Certificate of Analysis | May 25, 2023 | A491542 |
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Chemical and Physical Properties
| Solubility | souble in water |
|---|---|
| Sensitivity | Moisture sensitive |
Solution Calculators
Reviews
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