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Acetylcholine chloride - 10mM in DMSO, high purity , CAS No.60-31-1

    Grade & Purity:
  • 10mM in DMSO
In stock
Item Number
A425009
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A425009-1ml
1ml
Available within 4-8 weeks(?)
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$69.90

Endogenous neurotransmitter active at acetylcholine receptors

Basic Description

Synonyms ACETYLCHOLINE CHLORIDE | 60-31-1 | Miochol | Acecoline | Acecholin | ACH chloride | Chloroacetylcholine | Arterocoline | Ovisot | Azetylcholinchlorid | 2-(Acetyloxy)-N,N,N-trimethylethanaminium chloride | MIOCHOL-E | Acetylcholinium chloride | O-Acetylcholine chloride | 2-Acetoxyeth
Specifications & Purity 10mM in DMSO
Biochemical and Physiological Mechanisms Endogenous neurotransmitter in both the central and peripheral nervous systems. Displays activity at the nicotinic and muscarinic acetylcholine receptors. Also implicated in numerous non-neuronal roles.
Storage Temp Protected from light,Store at -80°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description

Acetylcholine chloride, more commonly referred to as just acetylcholine, is a cholinergic neurotransmitter that can induce the opening of calcium channels, as well as act on nicotinic and muscarinic acetylcholine receptors. Acetylcholine plays an important role at many sites in the central nervous system. The compound has been shown to have ophthalmological uses and can be broken down quickly by choliesterases. Studies show that non-neuronal acetylcholine influences many basic cells functions, such as mitosis, cells differentiation, cytoskeletal organization, and cell to cell contact, among other functions.
A cholinergic neurotransmitter that can induce the opening of calcium channels

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organic nitrogen compounds
Class Organonitrogen compounds
Subclass Quaternary ammonium salts
Intermediate Tree Nodes Cholines
Direct Parent Acyl cholines
Alternative Parents Tetraalkylammonium salts  Carboxylic acid esters  Monocarboxylic acids and derivatives  Organopnictogen compounds  Organic oxides  Organic chloride salts  Hydrocarbon derivatives  Carbonyl compounds  Amines  
Molecular Framework Aliphatic acyclic compounds
Substituents Acyl choline - Tetraalkylammonium salt - Carboxylic acid ester - Monocarboxylic acid or derivatives - Carboxylic acid derivative - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic chloride salt - Organic salt - Organooxygen compound - Carbonyl group - Amine - Aliphatic acyclic compound
Description This compound belongs to the class of organic compounds known as acyl cholines. These are acylated derivatives of choline. Choline or 2-Hydroxy-N,N,N-trimethylethanaminium is a quaternary ammonium salt with the chemical formula (CH3)3N+(CH2)2OH.
External Descriptors quaternary ammonium salt

Associated Targets(Human)

CHRM1 Tclin Muscarinic acetylcholine receptor M1 (12690 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CHRM5 Tclin Muscarinic acetylcholine receptor M5 (4677 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CHRM3 Tclin Muscarinic acetylcholine receptor M3 (7750 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
GLP1R Tclin Glucagon-like peptide 1 receptor (111429 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Homo sapiens (32628 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
SLC5A7 Tchem High-affinity choline transporter (1462 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
APEX1 Tchem DNA-(apurinic or apyrimidinic site) lyase (38016 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CBX1 Tbio Chromobox protein homolog 1 (92434 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
TNNC1 Tclin Troponin, cardiac muscle (276 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Associated Targets(non-human)

ampC Beta-lactamase AmpC (62480 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Pseudomonas aeruginosa (123386 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Klebsiella pneumoniae (43867 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Staphylococcus aureus (210822 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Acinetobacter baumannii (41033 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Escherichia coli (133304 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Plasmodium falciparum (966862 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Cryptococcus neoformans (21258 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Candida albicans (78123 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
Trpc4 Short transient receptor potential channel 4 (1615 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
vpr Aberrant vpr protein (14595 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Names and Identifiers

IUPAC Name 2-acetyloxyethyl(trimethyl)azanium;chloride
INCHI InChI=1S/C7H16NO2.ClH/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1
InChIKey JUGOREOARAHOCO-UHFFFAOYSA-M
Smiles CC(=O)OCC[N+](C)(C)C.[Cl-]
Isomeric SMILES CC(=O)OCC[N+](C)(C)C.[Cl-]
WGK Germany 2
RTECS FZ9800000
Molecular Weight 181.66
Beilstein 3571875
Reaxy-Rn 3571875
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3571875&ln=

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Chemical and Physical Properties

Sensitivity Light and moisture sensitive
Melt Point(°C) 149-152°C
Molecular Weight 181.660 g/mol
XLogP3
Hydrogen Bond Donor Count 0
Hydrogen Bond Acceptor Count 3
Rotatable Bond Count 4
Exact Mass 181.087 Da
Monoisotopic Mass 181.087 Da
Topological Polar Surface Area 26.300 Ų
Heavy Atom Count 11
Formal Charge 0
Complexity 115.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 2

Citations of This Product

1. Li Qiwei, Chen Zaozao, Zhang Ying, Ding Shuang, Ding Haibo, Wang Luping, Xie Zhuoying, Fu Yifu, Wei Mengxiao, Liu Shengnan, Chen Jialun, Wang Xuan, Gu Zhongze.  (2023)  Imaging cellular forces with photonic crystals.  Nature Communications,  14  (1): (1-14). 
2. Guorui Chang, Sirui Li, Yunqi Wang, Qingxia Ran, Qing Tan, Shuli Gou, Haiying Du, Shuxia Xu.  (2024)  Cu-C3N4 nanoenzyme-based freezing-dried bioactive capsule integrated with 3D-printed smartphone platform for visual detection of organophosphorus pesticides paraoxon in scallion.  SENSORS AND ACTUATORS B-CHEMICAL,  398  (134584). 
3. Zhe Ling, Yang Tan, Xinjian Li, Ying Xie, Peng Wang, Yan Su, Qiang Yong.  (2023)  Elucidating multi-scale deconstruction of bamboo crystalline cellulose by novel acetylcholine chloride based deep eutectic solvents for enhanced enzymatic hydrolysis.  INDUSTRIAL CROPS AND PRODUCTS,  203  (117156). 
4. Yaxu Zhao, Lijun Liang, Wei Guo, Xiong Lu, Congyi Zhao, Faming Gao.  (2023)  The porous hollow cobalt-based oxides encapsulated with bimetallic PdAu Nanoparticles of electrochemical biosensor for highly sensitive pesticides detection.  NANOTECHNOLOGY,  34  (28): (285501). 
5. Zhou Liping, Lao Zishan, Fan Xinsheng, Hao Mengyang, Yang Yubing.  (2023)  Sulfur dioxide derivatives aggravated ovalbumin-induced asthma through targeting TRPV1 and tight junctions.  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,     
6. Wang Kai, Liang Yaping, Duan Manman, Che Wunan, He Lu.  (2023)  Chronic toxicity of broflanilide in Daphnia magna: changes in molting, behavior, and gene expression.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,  30  (19): (54846-54856). 
7. Haoge Cheng, Kaiyue Yang, Yuze Zhang, Shenglong Liao, Menghao Du, Jiaqi Li, Ning Ma, Mianqi Xue, Xinyue Zhang, Yapei Wang.  (2022)  A Novel Ionic Conductive Polyurethane Based on Deep Eutectic Solvent Continuing Traditional Merits.  ACS Applied Materials & Interfaces,  14  (46): (52402–52410). 
8. Hangjing Zhang, Tianyuan Gao, Lei Jiang, Xiangqi Meng, Jian Wang, Ning Ma, Hao Wei, Xinyue Zhang.  (2022)  Conductive and transparent poly (N-isopropylacrylamide) hydrogels with tunable LCST copolymerized by the green acrylamide-based deep eutectic solvent.  EUROPEAN POLYMER JOURNAL,  173  (111266). 
9. Zhiang Zhang, Jikun Jiang, Xiao (Xiao) Liu, Xin Wang, Luyao Wang, Yuankun Qiu, Zhanfei Zhang, Yiting Zheng, Xueyun Wu, Jianghu Liang, Congcong Tian, Chun-Chao Chen.  (2021)  Surface-Anchored Acetylcholine Regulates Band-Edge States and Suppresses Ion Migration in a 21%-Efficient Quadruple-Cation Perovskite Solar Cell.  Small,  18  (6): (2105184). 
10. Xinyue Dai, Kai Le, Fenglong Wang, Rubin Wei, Jiurong Liu, Yanyan Jiang, Hui Li.  (2020)  Single-Molecule Detection of Acetylcholine by Translating the Neuronal Signal to a Single Distinct Electronic Peak.  ACS Applied Bio Materials,  (10): (6888–6896). 
11. Zhijiao Tang, Zhengyi Chen, Gongke Li, Yuling Hu.  (2020)  Multicolor nitrogen dots for rapid detection of thiram and chlorpyrifos in fruit and vegetable samples.  ANALYTICA CHIMICA ACTA,  1136  (72). 
12. Benhong Xu, Huixing Deng, Xiaojing Zhang, Jingxiong Luo, Gaoxiao Zhang, Zaijun Zhang, Yuqiang Wang, Luchen Shan.  (2018)  A novel Danshensu/tetramethylpyrazine derivative induces vasorelaxation on rat aorta and exerts cardioprotection in dogs.  EUROPEAN JOURNAL OF PHARMACOLOGY,  818  (158). 
13. Lve Wang, Xu Chen, Changxia Liu, Wensheng Yang.  (2016)  Non-enzymatic acetylcholine electrochemical biosensor based on flower-like NiAl layered double hydroxides decorated with carbon dots.  SENSORS AND ACTUATORS B-CHEMICAL,  233  (199). 
14. Lingsha Cheng, Haoqian Wu, Xiaoying Cai, Youying Zhang, Siqi Yu, Yuanlong Hou, Zhe Yin, Qingyuan Yan, Qiong Wang, Taipeng Sun, Guangji Wang, Yonggui Yuan, Xueli Zhang, Haiping Hao, Xiao Zheng.  (2024)  A Gpr35-tuned gut microbe-brain metabolic axis regulates depressive-like behavior.  Cell Host & Microbe,     
15. Ziyu Gan, Jun Wang.  (2025)  A MOF-stabilized enzyme-based colorimetric biosensor integrated with pH test strips for portable detection of D-limonene in agricultural applications.  CHEMICAL ENGINEERING JOURNAL,  510  (161719). 
16. Bo Liu, Tingxuan Duan, Tianyu Wang, Xiuyan Ren, Guanghui Gao.  (2025)  Direct Ink-Writable Tough polyurethane acrylate elastomers for underwater intelligent monitoring Equipment.  CHEMICAL ENGINEERING JOURNAL,  509  (161448). 
17. Liu Xiaojie, Jiang Xi, Hu Jing, Ding Mingxing, Lee Sang Ki, Korivi Mallikarjuna, Qian Yongdong, Li Ting, Wang Lifeng, Li Wei.  (2024)  Exercise attenuates high-fat diet-induced PVAT dysfunction through improved inflammatory response and BMP4-regulated adipose tissue browning.  Frontiers in Nutrition,  11   
18. Ni Su, Kuangbing Wang, Xinran Li, Xiankuan Huo, Guobi Chai, Wu Fan, Qingzhao Shi, Mengya Lv, Shusheng Zhang, Jianping Xie, Ronghan Wei, Qidong Zhang, Qiyan Wang.  (2024)  Laser-induced stripping defect for highly selective electrochemical quantification of dopamine: Anti-interference from other catecholamine neurotransmitters.  TALANTA,  279  (126638). 
19. Shun Wang, Yaqing Han, Mengdi Su, Hao Wang, Yuze Chen, Baoling Huang, Qian Bai, Mengke Wang.  (2025)  Mediated self-assembled gold nanoclusters with mesoporous silica particles to boost fluorescence for enhanced on-site monitoring of organophosphate pesticides.  FOOD CHEMISTRY,  463  (141120). 
20. Wei Wei, Heng Yan-Yan, Wu Fei-Fei, Dong Hao-Yu, Zhang Peng-Fei, Li Jing-Xia, Liu Chun-Yan, Yang Bing-Jie, Fu Jia-Ning, Liang Xin-Yue.  (2024)  Sodium Tanshinone IIA Sulfonate alleviates vascular senescence in diabetic mice by modulating the A20-NFκB-NLRP3 inflammasome-catalase pathway.  Scientific Reports,  14  (1): (1-16). 
21. Tingxuan Duan, Bo Liu, Yiyan Gao, Guanghui Gao.  (2024)  Wet Spinning/UV Dual-Curing enabled 3D printable fiber for intelligent electronic devices.  CHEMICAL ENGINEERING JOURNAL,  498  (155186). 

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