32 Citations
F1501085Determine the necessary mass, volume, or concentration for preparing a solution.
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Basic Description
| Product Name | α-Chymotrypsin from bovine pancreas(TLCK Treated) - ≥45 units/mg protein, high purity , CAS No.9004-07-3 |
|---|---|
| Synonyms | N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate | Chymotrypsin Substrate I, Colorimetric | N-(3-Carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide | AKOS024350585 | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]ami |
| Grade | EnzymoPure™ |
| Product Description |
Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues. |
| Specifications & Purity | EnzymoPure™, ≥45 units/mg protein |
| Biochemical and Physiological Mechanisms | A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide c |
| Accession # | P00766 |
Storage and Shipping
| Concentration | ≥45 units/mg protein |
|---|---|
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| CAS | 9004-07-3 |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Protein sample preparation process
Categories: Technical articles
Citations of This Product
| 1. Fenghong Deng, Shunjing Luo, Xiuting Hu, Chengmei Liu. (2024) Preparation, structural characterization and properties of feruloyl oligosaccharide–rice protein hydrolysate conjugates. FOOD RESEARCH INTERNATIONAL, 176 (113844). |
| 2. Junjie Lin, Chun Cui, Yunzi Feng. (2023) Preparation and Kokumi Properties of N-Acetyl-Val/Leu/Ile/Met/Phe in the Presence of Acetic Acid and Amino Acid: A Commercially Available Transglutaminase and Protease A 2SD. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 71 (45): (17235–17242). |
| 3. Wei Jia, Xin Wang. (2023) 3-Chloropropane-1,2-diol exposure adversely influenced the bio-accessibility signatures of digested infant foods by suppressing the destabilization of α-lactalbumin and d-aspartate oxidase in a dose-dependent manner. FOOD CHEMISTRY, 427 (136729). |
| 4. Peng Zhao, Rui Wang, Jun Xiang, Jinwei Zhang, Xiaodong Wu, Chaojian Chen, Gongyan Liu. (2023) Antibacterial, antiviral, and biodegradable collagen network mask for effective particulate removal and wireless breath monitoring. JOURNAL OF HAZARDOUS MATERIALS, 456 (131654). |
| 5. Songgang Xia, Jian Song, Ku Li, Tingting Hao, Chengxin Ma, Shuo Shen, Xiaoming Jiang, Changhu Xue, Yong Xue. (2023) Yeast protein-based meat analogues: Konjac glucomannan induces the fibrous structure formation by modifying protein structure. FOOD HYDROCOLLOIDS, 142 (108798). |
| 6. Shuqi Guo, Qiang Zhao, Yuze Li, Shanshan Chu, Falin He, Xiangxiang Li, Ning Sun, Wansong Zong, Rutao Liu. (2023) Potential toxicity of bisphenol A to α-chymotrypsin and the corresponding mechanisms of their binding. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 285 (121910). |
| 7. Okon Johnson Esua, Da-Wen Sun, Jun-Hu Cheng, Huifen Wang, Chen Chen. (2022) Hybridising plasma functionalized water and ultrasound pretreatment for enzymatic protein hydrolysis of Larimichthys polyactis: Parametric screening and optimization. FOOD CHEMISTRY, 385 (132677). |
| 8. Tian Quan, Dandan Wang, Lijuan Yang, Shaochi Liu, Yongqin Tao, Junji Wang, Linlin Deng, Xun Kang, Kailian Zhang, Zhining Xia, Die Gao. (2022) Effective extraction methods based on hydrophobic deep eutectic solvent coupled with functional molecularly imprinted polymers: Application on quercetagetin extraction from natural medicine and blood. MICROCHEMICAL JOURNAL, 174 (107076). |
| 9. Hongxia Tan, Hongyuan Zhou, Ting Guo, Jiaxin Li, Chi Zhang, Shuo Wang, Yuhao Zhang, Liang Ma. (2022) Zein structure and its hidden zearalenone: Effect of zein extraction methods. FOOD CHEMISTRY, 374 (131563). |
| 10. Yuqiu Zi, Dawei Xu, Cong Li, Fei Qu, Xian-En Zhao. (2021) Aggregation-enhanced emission of metal nanoclusters triggered by peptide self-assembly and application in chymotrypsin inhibitor screening. SENSORS AND ACTUATORS B-CHEMICAL, 345 (130243). |
| 11. Hongxia Tan, Hongyuan Zhou, Ting Guo, Yuhao Zhang, Jiaxin Li, Chi Zhang, Liang Ma. (2021) Effect of temperature and pH on the conversion between free and hidden zearalenone in zein. FOOD CHEMISTRY, 360 (130001). |
| 12. Jin Zhang, Chunsheng Xiao, Xi Zhang, Yandai Lin, Huanghao Yang, Yu Shrike Zhang, Jianxun Ding. (2021) An oxidative stress-responsive electrospun polyester membrane capable of releasing anti-bacterial and anti-inflammatory agents for postoperative anti-adhesion. JOURNAL OF CONTROLLED RELEASE, 335 (359). |
| 13. Zhang Huaiyu, Zhang Jiayu, Liu Yilun, Jiang Yang, Li Zhongmin. (2021) Molecular Targeted Agent and Immune Checkpoint Inhibitor Co-Loaded Thermosensitive Hydrogel for Synergistic Therapy of Rectal Cancer. Frontiers in Pharmacology, 12 |
| 14. Zong-Bo Xie, Lei-Han Fu, Jia Meng, Jin Lan, Zhi-Yu Hu, Zhang-Gao Le. (2020) Efficient biocatalytic strategy for one-pot Biginelli reaction via enhanced specific effects of microwave in a circulating reactor. BIOORGANIC CHEMISTRY, 101 (103949). |
| 15. Dandan Hu, Yufang Hu, Tianyu Zhan, Yudi Zheng, Pingjian Ran, Xinda Liu, Zhiyong Guo, Wenting Wei, Sui Wang. (2020) Coenzyme A-aptamer-facilitated label-free electrochemical stripping strategy for sensitive detection of histone acetyltransferase activity. BIOSENSORS & BIOELECTRONICS, 150 (111934). |
| 16. Ding Ding, Bowei Du, Chao Zhang, Fakhar Zaman, Yaqin Huang. (2019) Isolation and identification of an antioxidant collagen peptide from skipjack tuna (Katsuwonus pelamis) bone. RSC Advances, 9 (46): (27032-27041). |
| 17. Ruru Chen, Lian Zhou, Hui Yang, Hailing Zheng, Yang Zhou, Zhiwen Hu, Bing Wang. (2019) Degradation Behavior and Immunological Detection of Silk Fibroin Exposure to Enzymes. ANALYTICAL SCIENCES, |
| 18. Yiping Chen, Jing Cao, Xiaoxue Jiang, Zhizhen Pan, Nanyan Fu. (2018) A sensitive ratiometric fluorescence probe for chymotrypsin activity and inhibitor screening. SENSORS AND ACTUATORS B-CHEMICAL, 273 (204). |
| 19. Rong Wang, Fanjun Zhang, Weiwei Lin, Wenkai Liu, Jiehua Li, Feng Luo, Yaning Wang, Hong Tan. (2018) Shape Memory Properties and Enzymatic Degradability of Poly(ε-caprolactone)-Based Polyurethane Urea Containing Phenylalanine-Derived Chain Extender. MACROMOLECULAR BIOSCIENCE, 18 (6): (1800054). |
| 20. Meng Wang, Jun Xing, Yu-Tang Sun, Ling-Xiang Guo, Bao-Ping Lin, Hong Yang. (2018) Thiol–ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis. RSC Advances, 8 (22): (11843-11849). |
| 21. Shu Peng, Yu-Chen Pan, Yaling Wang, Zhe Xu, Chao Chen, Dan Ding, Yongjian Wang, Dong-Sheng Guo. (2017) Sequentially Programmable and Cellularly Selective Assembly of Fluorescent Polymerized Vesicles for Monitoring Cell Apoptosis. Advanced Science, 4 (11): (1700310). |
| 22. Shi-Guo Zhang, Zong-Bo Xie, Lian-Sheng Liu, Meng Liang, Zhang-Gao Le. (2017) Synthesis of 2,3-dihydroquinazolin-4(1H)-ones catalyzed by α-chymotrypsin. CHINESE CHEMICAL LETTERS, 28 (101). |
| 23. Xuezhou Li, Jianxun Ding, Zhengzheng Zhang, Modi Yang, Jiakuo Yu, Jincheng Wang, Fei Chang, Xuesi Chen. (2016) Kartogenin-Incorporated Thermogel Supports Stem Cells for Significant Cartilage Regeneration. ACS Applied Materials & Interfaces, 8 (8): (5148–5159). |
| 24. Song Zhiming, Shi Bo, Ding Jianxun, Zhuang Xiuli, Zhang Xiaonan, Fu Changfeng, Chen Xuesi. (2015) A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics. Science China-Chemistry, 58 (7): (1159-1168). |
| 25. Hai-Jie Zhang, Yi-Hui Lu, Yi-Juan Long, Qin-Long Wang, Xiao-Xiao Huang, Rui Zhu, Xi-Liang Wang, Li-Ping Liang, Ping Teng, Hu-Zhi Zheng. (2014) An aptamer-functionalized gold nanoparticle biosensor for the detection of prion protein. Analytical Methods, 6 (9): (2982-2987). |
| 26. Liping Liang, Yijuan Long, Haijie Zhang, Qinlong Wang, Xiaoxiao Huang, Rui Zhu, Ping Teng, Xiliang Wang, Huzhi Zheng. (2013) Visual detection of prion protein based on color complementarity principle. BIOSENSORS & BIOELECTRONICS, 50 (14). |
| 27. Ying Liu, Rutao Liu. (2012) The interaction of α-chymotrypsin with one persistent organic pollutant (dicofol): Spectroscope and molecular modeling identification. FOOD AND CHEMICAL TOXICOLOGY, 50 (3298). |
| 28. XiaoXiao HUANG, YiJuan LONG, HaiJie ZHANG, QinLong WANG, Rui ZHU, HuZhi ZHENG. (2012) Gold Nanoparticles as a Probe for Prion Determination via Resonance Light Scattering Method. ANALYTICAL SCIENCES, 28 (5): (475-479). |
| 29. Hai-Jie Zhang, Hu-Zhi Zheng, Yi-Juan Long, Geng-Fu Xiao, Ling-Yan Zhang, Qin-Long Wang, Mei Gao, Wen-Jun Bai. (2012) Gold nanoparticles as a label-free probe for the detection of amyloidogenic protein. TALANTA, 89 (401). |
| 30. Jun Huang, Gang Yang, Kewei Chen, Muying Du, Zsolt Zalán, Ferenc Hegyi, Jianquan Kan. (2024) Anti-fungal effects of lactic acid bacteria from pickles on the growth and sterigmatocystin production of Aspergillus versicolor. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 422 (110809). |
| 31. Wenjing Chu, Mengyu Yang, Zhiwei Shang, Jing Zhao, Yuling Xiao, Jing Pan, Xiaoqing Yi, Meihua Lin, Fan Xia. (2025) Machine Learning Assisted Nanofluidic Array for Multiprotein Detection. ACS Nano, 19 (9): (8539-8551). |
| 32. Kanzhen Liu, Cuiping Pang, Qinghua Li, Jianghua Li, Guocheng Du, Guoqiang Zhang. (2025) Step Enzymatic Hydrolysis and In Silico Screening-Assisted Preparation of Bioactive Peptides from Abalone. Foods, 14 (7): (1209). |
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