Basic Description

Specifications & Purity	tablet, To prepare 5 mL
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	SIGMAFAST 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections. Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Benzenoids
  - Class Benzene and substituted derivatives
    - Subclass Biphenyls and derivatives
      - Level5 Benzidines
        Level6 3,3'-disubstituted benzidines

Kingdom	Organic compounds
Superclass	Benzenoids
Class	Benzene and substituted derivatives
Subclass	Biphenyls and derivatives
Intermediate Tree Nodes	Benzidines
Direct Parent	3,3'-disubstituted benzidines
Alternative Parents	Aniline and substituted anilines Primary amines Organopnictogen compounds Hydrocarbon derivatives
Molecular Framework	Aromatic homomonocyclic compounds
Substituents	3,3'-disubstituted benzidine - Aniline or substituted anilines - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Primary amine - Organonitrogen compound - Amine - Aromatic homomonocyclic compound
Description	This compound belongs to the class of organic compounds known as 3,3'-disubstituted benzidines. These are organic compounds containing a benzidine skeleton, which is substituted only at the 3- and 3'-positions.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Names and Identifiers

IUPAC Name	4-(3,4-diaminophenyl)benzene-1,2-diamine
INCHI	InChI=1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
InChIKey	HSTOKWSFWGCZMH-UHFFFAOYSA-N
Smiles	C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N
Isomeric SMILES	C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N
WGK Germany	1
RTECS	DV8750000
Molecular Weight	214.27
Beilstein	1212988
Reaxy-Rn	1212988
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1212988&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Chemical and Physical Properties

Melt Point(°C)	176-180°C
Molecular Weight	214.270 g/mol
XLogP3	0.900
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Exact Mass	214.122 Da
Monoisotopic Mass	214.122 Da
Topological Polar Surface Area	104.000 Å²
Heavy Atom Count	16
Formal Charge	0
Complexity	209.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Documents & Articles

Peroxidase Substrate Detection System

Categories: Technical articles

Tags:

HRP Immunoassay reagents and substrates Peroxidase TMB DAB ABTS Enzyme inhibitors and substrates Chromogenic substrates and chemiluminescence substrates

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Yanan Jiang, Yanbin Yun, Lihua Wang, Min He, Zhi Qiu, ZuYu Li. (2025) Achieving high-performance electrochemical CO2 reduction using metal-polybenzimidazole coordination anion exchange membranes. SEPARATION AND PURIFICATION TECHNOLOGY, 365 (132638).
2. Xinglin Tao, Peng Yang, Zhaoqi Liu, Siyao Qin, Jun Hu, Zhao-Xia Huang, Xiangyu Chen, Jin-Ping Qu. (2024) Acid-Doped Pyridine-Based Polybenzimidazole as a Positive Triboelectric Material with Superior Charge Retention Capability. ACS Nano, 18 (5): (4467-4477).
3. Fang Shi, Yong-Sheng Gao, Shu-Mei Han, Cheng-Suo Huang, Qing-Sheng Hou, Xiao-wen Wen, Ben-Shi Wang, Zhen-Yu Zhu, Lei Zou. (2024) Allulose mitigates chronic enteritis by reducing mitochondria dysfunction via regulating cathepsin B production. INTERNATIONAL IMMUNOPHARMACOLOGY, 129 (111645).
4. Wu Di, Song Qingzhi, Cui Deliang, Li Yanlu, Wang Qilong, Yu Haohai, Lian Gang. (2025) Aromatic polyamine-grafted and nitrogen-doped graphene anodes boosting surface-dominated sodium storage. Advanced Composites and Hybrid Materials, 8 (2): (1-12).
5. Liu Menglong, Wang Ying, Xiao Jingyi, Liu Yiyao, Ren Yi, Gao Xue. (2024) Colorimetric immunoassay of furazolidone metabolites based on iron-copper bimetallic organic framework nanoenzyme. MICROCHIMICA ACTA, 191 (10): (1-10).
6. Yiming Xiao, Haoran Chen, Xiaoyu Shen, Yuanyuan Li, Lei Zhang, Penggao Cheng, Huaiyuan Han, Na Tang. (2024) Construction of wide-temperature-range proton exchange membrane by regulating proton transfer channels based on phosphate-functionalized carbon nanotubes. JOURNAL OF MEMBRANE SCIENCE, 709 (123077).
7. Cuicui Li, Tongtong Gao, Wanzhen Wu, Xinpu Zhou, Shuyu Zhang, Guangpeng Ma, Xianfeng Guan, Shuang Wang. (2025) Enhanced proton conductivity of main/side chain bi-sulfonated polybenzimidazoles via embedment of fluorinated modified MOF-801 for vanadium redox flow batteries. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 686 (722).
8. Haiqing Lu, Tongda Lei, Li Zhang, Shiyang Chen, Rouxi Chen, Xipeng Li, Yongheng Wang, Jian Zhu, Mingyi Chen, Kai Zhang, Qingqi Yang, Sen He, Jie Fan. (2024) Flexible polyaniline@carbon nanofiber membrane pH electrode for health care. MICROCHEMICAL JOURNAL, 200 (110436).
9. Zequan Huang, Danyi Zhu, Mingjie Ma, Bing Zhao, Jiazhen Liang, Liang Zhang, Chao Chen, Mengjiao Liu, Congjie Gao, Fei Huang, Lixin Xue. (2024) Highly Efficient and Durable Water Electrolysis at High KOH Concentration Enabled by Cationic Group-Free Ion Solvating Membranes in Free-standing Gel Form. Small, (2408159).
10. Yufei Gao, Chunxue Wang, Han Ye, Ying Zhang. (2024) Highly ion-selective sulfonated poly (4,4′-diphenylether-5,5′-bibenzimidazole) membranes for vanadium redox flow battery. Journal of Experimental Nanoscience,
11. Xiaokeya Yasen, Renaguli Aikebaier, Atiguli Maimaiti, Munire Mushajiang. (2024) IL-33/soluble ST2 axis is associated with radiation-induced cardiac injury. Open Life Sciences, 19 (1):
12. Houwen Hu, Haoming Xing, Linfan Wang, Sineng Gao, Yihao Zhang, Liwei Gao, Guoqiang Guo, Chenjie Gu, Da Chen. (2024) Lateral flow assay with dual-emission nitrogen-doped carbon dots for ratiometric fluorescent determination of nitrite in food. MICROCHEMICAL JOURNAL, 205 (111247).
13. Cuicui Li, Tongtong Gao, Wanzhen Wu, Xinpu Zhou, Shuyu Zhang, Guangpeng Ma, Xianfeng Guan, Shuang Wang. (2024) Low Permeability Membrane Regulated by Main/Side-Chain Bisulfonated Polybenzimidazole and CAU-10-H for Vanadium Redox Flow Batteries. ACS Applied Energy Materials, 7 (17): (7534-7544).
14. Bowen Zheng, Chengwei Deng, Ruoyin Luo, Shaojie Gao, Feng Ji, Dong Wang. (2024) Mechanically strengthened polybenzimidazole membrane via a two-step crosslinking strategy for high-temperature proton exchange membrane fuel cell. JOURNAL OF POWER SOURCES, 603 (234369).
15. Shuangjiang Li, Yi Liu, Lan Shi, Tianding Hu, Mengrui Li, Churu Zhang, Shaoyun Shan, Yunfei Zhi. (2024) Salen-based ionic polymers as efficient heterogeneous catalysts for CO2 cycloaddition. Journal of Environmental Chemical Engineering, 12 (111989).
16. Weiyu Zhang, Wenwen Wang, Dong Xie, Jie Li, Hong Li, Jingxuan Dai, Yi Tang, Tianqi Yang, Weiyi Jin, Panpan Zhou, Chenliang Gong. (2024) Sulfonating and polyhedral oligomeric silsesquioxane crosslinking in one step to build multiple proton pathways for proton exchange membrane fuel cells over a wide temperature range. JOURNAL OF POWER SOURCES, 623 (235410).
17. Zi-Lu Zhang, Jia-Hui Xin, Ping Fu, Wan-Long Li, Wan-Ting Lin, Xiao-Wei Luo, Chang Liu, Zhi-Kang Xu, Ling-Shu Wan. (2025) Thermally stable thin-film composite nanofiltration membranes derived from 3,3′-diaminobenzidine. JOURNAL OF MEMBRANE SCIENCE, 713 (123386).
18. Jiaqi Ji, Tianqi Yang, Weiyu Zhang, Jie Li, Hong Li, Yi Tang, Weiyi Jin, Yiming Sun, Keda Li, Wei Li, Xiaoquan Ding, Chenliang Gong. (2025) Triazine-rich covalent organic framework composited proton exchange membranes for flexible operating temperature and enhanced long-term stability fuel cells. JOURNAL OF POWER SOURCES, 632 (236351).

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SKU	Size	Availability	Price
D755513-5T	5T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$45.90
D755513-50T	50T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$225.90
D755513-5×50T	5×50T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$836.90

3,3′-Diaminobenzidine tablets - tablet, To prepare 5 mL, high purity , CAS No.91-95-2